| Catalog | IV0113 |
| Product Name | InVivoPro Anti-Mouse Ly6G/Ly6C (Gr-1) in vivo antibody, Clone RB6-8C5 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Rat IgG2b |
| Clone | RB6-8C5 |
| Target | Ly6G/Ly6C |
| Other Names | Ly-6G/LY-6C, Gr-1 |
| Isotype Control Catalog Code | IV0106 or Rat IgG2b Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Rat |
| How much antibody to use in vivo | 200-500 μg per mouse; or 10-20 mg/kg. This range is based off the most recent publication data using the RB6-8C5 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Citations of the same Clone | 200 |
| Background | Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C. |
| Applications | In vivo depletion of Gr-1+ myeloid cells, Flow cytometry, Immunohistochemistry(Paraffin and Frozen), Western blot |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
