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Cat. Number
IV0104
Chemical Name
IV0104 InVivoPro Anti-Mouse CD8α in vivo antibody, Clone 2.43
References

Product Details

CD8 is part of the Ig superfamily that expresses primarily as CD8α homodimers. CD8α is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8β. Clone 2.43 recognizes an epitope on mouse CD8a.

Specifications

CatalogIV0104
Product NameInVivoPro Anti-Mouse CD8α in vivo antibody, Clone 2.43
Size1mg/5mg/25mg/50mg/100mg
IsotypeRat IgG2b
Clone2.43
TargetCD8α
Other NamesCD8 alpha chain,Ly 2.2,Ly-35
Isotype Control Catalog CodeIV0106 or Rat IgG2b Isotype Control 
Dilution BufferPBS, pH 7.2, contains no stabilizers or preservatives
ReactivityMouse
Host SpeciesRat
How much antibody to use in vivo200-500 μg per mouse; or 10 mg/kg. This range is based off the most recent publication data using the 2.43 clone in vivo. Each investigator should  determine their own optimal working dilution for specific applications.
BackgroundCD8 is part of the Ig superfamily that expresses primarily as CD8α homodimers. CD8α is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8β. Clone 2.43 recognizes an epitope on mouse CD8a.
ApplicationsIn vivo CD8+ T cell depletion, Flow cytometry
Purificationprotein A or G
StorageThis antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles.
Shipping2-8°C with blue ice
ConcentrationLot specific, generally ≥ 5.0 mg/ml
Shelf Life12 months from the date of receipt if stored as recommended
FormulationPBS Buffer, PH 7.2, with no carrier protein, or preservatives.
Sterility0.2 μM filtered
Endotoxin≤ 1.0 EU/mg, by the LAL method
Purity99%

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