IV0121 InVivoPro Anti-Mouse CD3ε in vivo antibody, Clone 145-2C11_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

CD3ε is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Specifications

Catalog	IV0121
Product Name	InVivoPro Anti-Mouse CD3ε in vivo antibody, Clone 145-2C11
Size	1mg/5mg/25mg/50mg/100mg
Isotype	Armenian Hamster IgG
Clone	145-2C11
Target	CD3ε
Other Names	T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e
Isotype Control Catalog Code	Armenian Hamster IgG Isotype Control
Dilution Buffer	PBS, pH 7.2, contains no stabilizers or preservatives
Reactivity	Mouse
Host Species	Armenian Hamster
How much antibody to use in vivo	2-5 μg/mL for in vitro; 200 μg per mouse. This range is based off the most recent publication data using the 145-2C11 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.
Background	CD3ε is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Applications	In vivo T cell depletion, in vitro T cell stimulation, Flow cytometry, Western blot, Immunohistochemistry(Frozen)
Purification	protein A or G
Storage	This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles.
Shipping	2-8°C with blue ice
Concentration	Lot specific, generally ≥ 5.0 mg/ml
Shelf Life	12 months from the date of receipt if stored as recommended
Formulation	PBS Buffer, PH 7.2, with no carrier protein, or preservatives.
Sterility	0.2 μM filtered
Endotoxin	≤ 1.0 EU/mg, by the LAL method
Purity	99%