IV0116 InVivoPro Anti-Mouse CD40 in vivo antibody, Clone FGK45_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD154) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases (4). Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

Specifications

Catalog	IV0116
Product Name	InVivoPro Anti-Mouse CD40 in vivo antibody, Clone FGK45
Size	1mg/5mg/25mg/50mg/100mg
Isotype	Rat IgG2a
Clone	FGK45
Target	CD40
Other Names	CD40, Bp50, TNFRSF5
Isotype Control Catalog Code	IV0105 or Rat IgG2a Isotype Control
Dilution Buffer	PBS, pH 7.2, contains no stabilizers or preservatives
Reactivity	Mouse
Host Species	Rat
How much antibody to use in vivo	200-500 μg per mouse; or 10-20 mg/kg. This range is based off the most recent publication data using the FGK45 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.
Background	CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD154) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases (4). Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Applications	In vivo CD40 activation, in vitro B cell stimulation/activation, Flow cytometry
Purification	protein A or G
Storage	This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles.
Shipping	2-8°C with blue ice
Concentration	Lot specific, generally ≥ 5.0 mg/ml
Shelf Life	12 months from the date of receipt if stored as recommended
Formulation	PBS Buffer, PH 7.2, with no carrier protein, or preservatives.
Sterility	0.2 μM filtered
Endotoxin	≤ 1.0 EU/mg, by the LAL method
Purity	99%