IV0120 InVivoPro Anti-Mouse CD28 in vivo antibody, Clone 37.51_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

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Cat. Number

IV0120

Chemical Name

IV0120 InVivoPro Anti-Mouse CD28 in vivo antibody, Clone 37.51

Qty 1

5mg

Qty 2

50mg

References

Catalog: IV0120

Cost advantage to: BE0015-1

Clone: 37.51

Isotype: Syrian Hamster IgG

Citations of the same Clone: 175

Product Details

CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. It is expressed on the cell surface as disulfide-linked homodimer or as monomer. CD28 is the receptor for B7.1 (CD80) and B7.2 (CD86). When activated by Toll-like receptor ligands, the B7.1 expression is upregulated in antigen presenting cells (APCs). The B7.2 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. Stimulation through CD28 in addition to the TCR can provide a potent co-stimulatory signal to T cells for the production of various interleukins (IL-2 and IL-6 in particular). CD28 possesses an intracellular domain with several residues that are critical for its effective signalling. CD28 also contains two proline-rich motifs that are able to bind SH3-containing proteins.CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-gamma.

Specifications

Catalog	IV0120
Product Name	InVivoPro Anti-Mouse CD28 in vivo antibody, Clone 37.51
Size	1mg/5mg/25mg/50mg/100mg
Isotype	Syrian Hamster IgG
Clone	37.51
Target	CD28
Other Names	MGC138290, Tp44, T44
Isotype Control Catalog Code	Syrian Hamster IgG Isotype Control
Dilution Buffer	PBS, pH 7.2, contains no stabilizers or preservatives
Reactivity	Mouse
Host Species	Syrian Hamster
How much antibody to use in vivo	5 μg/mL for in vitro; 100 μg per mouse. This range is based off the most recent publication data using the 37.51 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.
Background	CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. It is expressed on the cell surface as disulfide-linked homodimer or as monomer. CD28 is the receptor for B7.1 (CD80) and B7.2 (CD86). When activated by Toll-like receptor ligands, the B7.1 expression is upregulated in antigen presenting cells (APCs). The B7.2 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. Stimulation through CD28 in addition to the TCR can provide a potent co-stimulatory signal to T cells for the production of various interleukins (IL-2 and IL-6 in particular). CD28 possesses an intracellular domain with several residues that are critical for its effective signalling. CD28 also contains two proline-rich motifs that are able to bind SH3-containing proteins.CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-gamma.
Applications	In vivo CD28 blockade, in vitro T cell stimulation/activation, Flow cytometry, Western blot
Purification	protein A or G
Storage	This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles.
Shipping	2-8°C with blue ice
Concentration	Lot specific, generally ≥ 5.0 mg/ml
Shelf Life	12 months from the date of receipt if stored as recommended
Formulation	PBS Buffer, PH 7.2, with no carrier protein, or preservatives.
Sterility	0.2 μM filtered
Endotoxin	≤ 1.0 EU/mg, by the LAL method
Purity	99%