HA11612 肝素酶II Recombinant Heparinase II 52227-76-6_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

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Cat. Number

HA11612

Chemical Name

HA11612 肝素酶II Recombinant Heparinase II 52227-76-6

CAS Number

52227-76-6

Mol. Weight

87 kDa

Qty 1

0.1IU

Qty 2

0.25IU

Appearance

Lyophilized powder

Application Notes

≥ 95%

Storage condition

-20°C

Stability

2年

References

多糖裂解酶（Polysaccharide Lyase）是一类主要作用于阴离子多糖,通过β消除机制降解糖苷键形成在232nm波长有吸收的C4-C5双键的不饱和寡糖。作为一类生物技术工具常用于多糖的纯度和结构分析，以及寡糖的制备。肝素酶可选择地剪切硫酸化肝素聚糖中葡萄糖胺和糖醛酸之间α(1-4)糖苷键,三种不同的肝素酶(酶Ⅰ、酶Ⅱ、酶Ⅲ)具有非常不同的专属性。
上海惠诚生物提供三种肝素酶(heparinase)，分别是Recombinant Heparinase I,Heparinase II和Heparinase III，咨询订购热线：021-60498804

Appearance:	Lyophilized powde
Purity：	≥ 95%
Specific Activity ：	≥ 3.4 IU/mg protein
Stabilizer：	None or BSA
Protein concentration (BCA assay):	1.0 mg/ml
Activity (using heparin as substrate):	3.8 IU/mg
Assay Buffer:	100 mM MOPS, 5 mM CaCl 2 , pH 7.0

将200μl肝素溶液（在测定缓冲液中为2.5 mg/ml）加入含有50μl重组蛋白的孔中肝素酶II（在测定缓冲液中新制备的5μg），并在动力学中监测232nm处的吸光度在M5 Microplate Reader

用线性回归分析对数据进行拟合，以获得对计算比活度：

HA11785 肝素酶I Recombinant Heparinase I
0.05IU 1580元
0.1IU 2850元
Source: E. coli-derived Gln22-Arg384, with an N-terminal 6-His tag
SDS-PAGE: 43.6 kDa, reducing conditions
Appearance: Supplied as a 0.2 μm filtered frozen solution in 20 mM Tris, 150 mM NaCl, 2.5mM CaCl2, pH 7.4.

HA11612 肝素酶II Recombinant Heparinase II
0.1IU 1580元
0.25IU 3570元
Protein concentration (BCA assay): 0.4 mg/ml
Activity (using heparin as substrate): 2.7 IU/mg
200 μl of heparin solution (2.5 mg/ml in assay buffer) was added into a well containing 50 μl of recombinant
Heparinase II (2.4μg freshly prepared in assay buffer), and the absorbance at 232nm was monitored in a kinetic mode (in 15 s intervals for 2 min) at 37℃ performed in a SpectraMax M5 Microplate Reader (Molecular Devices,USA).

HA11587 肝素酶III Recombinant Heparinase III
0.5IU 1580元
1.0IU 2850元
SDS-PAGE: 75 kDa, reducing conditions
Source: E. coli-derived Gln25-Pro659, with an N-terminal 6-His tag
Appearance: Supplied as a 0.2 μm filtered frozen solution in 50 mM sodium phosphate, pH 7.6.