HA11785 肝素酶I Recombinant Heparinase I 52227-76-6_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

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Cat. Number

HA11785

Chemical Name

HA11785 肝素酶I Recombinant Heparinase I 52227-76-6

CAS Number

52227-76-6

Mol. Weight

43.6 kDa

Qty 1

0.05IU

Qty 2

0.1IU

Appearance

powder or liquid

Application Notes

90%

Synonym

肝素酶I

Storage condition

-20℃

Stability

2年

References

肝素酶可选择地剪切硫酸化肝素聚糖中葡萄糖胺和糖醛酸之间α(1-4)糖苷键,三种不同的肝素酶(酶Ⅰ、酶Ⅱ、酶Ⅲ)具有非常不同的专属性。上海惠诚生物提供三种肝素酶(heparinase)，分别是Recombinant Heparinase I,Heparinase II和Heparinase III,肝素酶产品咨询订购上海惠诚生物021-60498804

Appearance：	powder or liquid
Purity：	90%
Protein concentration (BCA assay)	1.0 mg/ml
Activity (using heparin as substrate)	0.40 IU/mg
Assay Buffer	100 mM MOPS, 5 Mm CaCl 2 , pH 7.0

将200µl 2.5 mg/ml肝素（在测定缓冲液中）加入到含有50µl重组肝素酶I的孔中（在测定缓冲液中新制备的10µg），并立即记录232nm处的吸光度，不振荡在SpectraMax M5微孔板阅读器（MolecularDevices，USA）。用线性回归分析对数据进行拟合以获得初始反应速率（斜率dA/dt，min-1）来计算比活性：

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HA11785 Recombinant Heparinase I
0.05IU 1580元
0.1IU 2850元
Source: E. coli-derived Gln22-Arg384, with an N-terminal 6-His tag
SDS-PAGE: 43.6 kDa, reducing conditions
Appearance: Supplied as a 0.2 μm filtered frozen solution in 20 mM Tris, 150 mM NaCl, 2.5mM CaCl2, pH 7.4.

HA11612 Recombinant Heparinase II
0.1IU 1580元
0.25IU 3570元
Protein concentration (BCA assay): 0.4 mg/ml
Activity (using heparin as substrate): 2.7 IU/mg
200 μl of heparin solution (2.5 mg/ml in assay buffer) was added into a well containing 50 μl of recombinant
Heparinase II (2.4μg freshly prepared in assay buffer), and the absorbance at 232nm was monitored in a kinetic mode (in 15 s intervals for 2 min) at 37℃ performed in a SpectraMax M5 Microplate Reader (Molecular Devices,USA).

HA11587 Recombinant Heparinase III
0.5IU 1580元
1.0IU 2850元
SDS-PAGE: 75 kDa, reducing conditions
Source: E. coli-derived Gln25-Pro659, with an N-terminal 6-His tag
Appearance: Supplied as a 0.2 μm filtered frozen solution in 50 mM sodium phosphate, pH 7.6.