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Cat. Number
066204435084260
Chemical Name
Colorimetric COX (ovine) Inhibitor Screening Assay Kit
References
Stability 1 year
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25 531-540 (1983).

760111-96WELL
96-Well Solid Plate (Colorimetric Assay)
Colorimetric COX Assay COX-1 (ovine) 1 ea
Colorimetric COX Assay COX-2 (ovine) 1 ea
Colorimetric COX Assay Arachidonic Acid (substrate) 1 ea
Colorimetric COX Assay Buffer (10X) 1 ea
Colorimetric COX Assay Potassium Hydroxide 1 ea
Colorimetric COX Assay Heme 1 ea
Colorimetric COX Assay Colorimetric Substrate 1 ea
Size Global Purchasing
96 wells  

Description

The Colorimetric COX Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.1 Inhibition of COX activity, measured by TMPD oxidation, by a variety of selective and nonselective inhibitors, shows changed potencies similar to those observed with other in vitro methods. The Colorimetric COX Inhibitor Screening assay includes both ovine COX-1 and COX-2 enzymes in order to screen isozyme-specific inhibitors. The Cayman Colorimetric COX Assay is a time saving tool for screening vast numbers of inhibitors.

1 Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25 531-540 (1983).

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