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Cat. Number

066209622725824

Chemical Name

CFSE Cell Division Assay Kit

References

Stability	1 year
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Parish, C.R., Glidden, M.H., Quah, B.J.C., et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr Protoc Immunol 4.9.1-4.9.13 (2009).

ulic, S., Panic, L., Ðikic, I., et al. Deregulation of cell growth and malignant transformation. Croat Med J 46(4) 622-638 (2005).

Lyons, A.B. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J Immunol Methods 243 147-154 (2000).

10009853-100test
Cell-Based Assay Buffer Tablet	4 ea
CFSE Stock Solution	3 × 1 ea

Size	Global Purchasing
100 tests

Description

Cell proliferation is controlled by growth factors that bind to receptors on the cell surface, which in turn connect to signaling molecules. These molecules activate transcription factors which bind to DNA to turn on or off the production of proteins which result in cell division. Dysfunction of any step in this regulatory cascade causes abnormal cell proliferation, which underlies many human pathological conditions.¹ The mechanisms underlying alterations in cell cycle progression are crucial to understanding many human diseases, most notably cancer. Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) is a novel cell-tracing fluorescent dye used to examine the proliferative activity of cells by the labeling of a parent generation and the inheritance of the label by daughter generations. CFDA-SE diffuses into cells, where the acetate groups on the molecule are cleaved to yield a highly fluorescent derivative (CFSE) that is retained in the cell and can be detected by flow cytometry. Cell division results in sequential halving of fluorescence, and up to eight divisions can be monitored before the fluorescence is decreased to the background fluorescence of unstained cells.² In addition, the stability of the CFSE labeling allows monitoring of cells over a period of a couple of weeks in vitro. Using this method, cells may be traced through successive generations without the use of radioactivity and complicated protocols. Since its first introduction in 1994, the flow cytometry analysis of CFSE-labeled cells has become the most informative experimental techniques for studying lymphocyte proliferation.³ Cayman’s CFSE Cell Division Assay Kit provides an easy to use format for labeling and tracing cells through successive cell divisions which can be used to study the induction and inhibition of cell division in any in vitro model. The kit contains sufficient reagents for labeling and analyzing 100 cell samples by flow cytometry. CFSE can also be combined with any fluorochrome compatible with fluorescein for use in flow cytometry.

1 ulic, S., Panic, L., Ðikic, I., et al. Deregulation of cell growth and malignant transformation. Croat Med J 46(4) 622-638 (2005).

2 Parish, C.R., Glidden, M.H., Quah, B.J.C., et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr Protoc Immunol 4.9.1-4.9.13 (2009).

3 Lyons, A.B. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J Immunol Methods 243 147-154 (2000).

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