| References |
| Stability |
1 year |
| Storage |
-80°C |
| Shipping |
Dry ice
in continental US; may vary elsewhere
|
| Specificity |
| Prostaglandin E1 |
100% |
| Prostaglandin E2 |
100% |
| Prostaglandin F2α |
100% |
| Prostaglandin F1α |
100% |
| Prostaglandin F3α |
51.3% |
| Prostaglandin E2 Ethanolamide |
44.0% |
| 6-keto Prostaglandin F1α |
43.6% |
| 8-iso Prostaglandin F2α |
38.4% |
| 8-iso Prostaglandin E2 |
28.5% |
| Prostaglandin D2 |
26.6% |
| 8-iso-2,3-dinor Prostaglandin F1α |
20.0% |
| Prostaglandin E3 |
9.5% |
| Thromboxane B2 |
5.0% |
| 12(S)-HHTrE |
0.25% |
| Leukotriene D4 |
0.2% |
| Arachidonic Acid |
<0.01% |
| Leukotriene B4 |
<0.01% |
| Leukotriene C4 |
<0.01% |
| Leukotriene E4 |
<0.01% |
| Misoprostol |
<0.01% |
| Misoprostol (free acid) |
<0.01% |
| Prostaglandin A1 |
<0.01% |
| Prostaglandin A2 |
<0.01% |
| Prostaglandin A3 |
<0.01% |
| Prostaglandin B1 |
<0.01% |
| 15-keto Prostaglandin E2 |
<0.01% |
| 13,14-dihydro-15-keto Prostaglandin F2α |
<0.01% |
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|
Background Reading
Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).
| Size |
Global Purchasing |
| 96 wells |
|
Description
Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) enzymes contain both cyclooxygenase and peroxidase activities. COX catalyzes the first step in the biosynthesis of prostaglandins (PGs), thromboxanes, and prostacyclins; the conversion of arachidonic acid to PGH2. It is now well established that there are two distinct isoforms of COX. Cyclooxygenase-1 (COX-1) is constitutively expressed in variety of cell types and is involved in normal cellular homeostasis. A variety of mitogenic stimuli such as phorbol esters, lipopolysaccharides, and cytokines lead to the induced expression of a second isoform of COX, cyclooxygenase-2 (COX-2). COX-2 is responsible for the biosynthesis of PGs under acute inflammatory conditions.1 This inducible COX-2 is believed to be the target enzyme for the anti-inflammatory activity of nonsteroidal anti-inflammatory drugs. The COX Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via enzyme immunoassay (EIA) using a broadly specific antibody that binds to all the major prostaglandin compounds. Thus, the Cayman COX assay is more accurate and reliable than an assay based on peroxidase inhibition. The Cayman COX Inhibitor Screening Assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.
1
Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).
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