EP2 Receptor (rat) STEP Reporter Assay Kit (Luminescence)_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

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Cat. Number

066106180207385

Chemical Name

EP2 Receptor (rat) STEP Reporter Assay Kit (Luminescence)

References

Synonyms	Prostaglandin E2 Receptor 2 PGE2 Receptor 2
Stability	1 year
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Negishi, M., Sugimoto, Y., and Ichikawa, A. Prostanoid receptors and their biological actions. Prog Lipid Res 32 417-434 (1993).

Sakata, D., Yao, C., and Narumiya, S. Prostaglandin E₂, an immunoactivator. J Pharmacol Sci 112 (2010).

Harris, S.G., Padilla, J., Koumas, L., et al. Prostaglandins as modulators of immunity. Trends Immunol 23(3) 144-150 (2002).

Yang, L., Yamagata, N., Yadav, R., et al. Cancer-associated immunodeficiency and dendritic cell abnormalities mediated by the prostaglandin EP₂ receptor. J Clin Invest 111(5) 727-735 (2003).

Sugimoto, Y., and Narumiya, S. Prostaglandin E receptors. J Biol Chem 282(16) 11613-11617 (2007).

Dey, I., Lejeune, M., and Chadee, K. Prostaglandin E₂ receptor distribution and function in the gastrointestinal tract. Br J Pharmacol 149 611-623 (2006).

McCullough, L., Wu, L., Haughey, N., et al. Neuroprotective function of the PGE₂ EP₂ receptor in cerebral ischemia. J Neurosci 24(1) 257-268 (2004).

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600340-1ea
96-Well Solid Plate (black) with lid
Purple Non-EIA Kit Box (480 Well)
SEAP Substrate (Luminescence)	15 mL
EP Receptor Assay Prostaglandin E2 Positive Control	20 µL
EP2 Receptor (rat) STEP Strip Plate

Size	Global Purchasing
100 tests

Description

The EP₂ receptor is one of 4 GPCRs that mediate the actions of PGE₂. The diverse effects of PGE₂ acting via EP₂ receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman’s EP₂ Receptor (rat) STEP Reporter Assay Kit (Luminescence) consists of a 96-well plate coated with both rat EP₂ receptor and Secreted Alkaline Phosphatase (SEAP) reporter constructs (EP₂ Receptor STEP Plate). Cells grown on the STEP complex will express EP₂ at the cell surface. Binding of agonists to EP₂ initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. Aliquots of media are removed at time intervals, beginning at approximately six hours, and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds regulating the activation of EP₂. PGE₂ is included in the kit for use as a positive control. The kit provides sufficient reagents to measure SEAP activity at three time points using the black plates included.

下一个：DPP (IV) Inhibitor Screening Assay Kit上一个：EP4 Receptor (rat) STEP Reporter Assay Kit (Luminescence)

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