HAT Inhibitor Screening Assay Kit_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

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Okumura, K., Mendoza, M., Bachoo, R.M., et al. PCAF modulates PTEN activity. J Biol Chem 281 26562-26568 (2006).

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Cheung, W.L., Briggs, D.B., and Allis, C.D. Acetylation and chromosomal functions. Curr Opin Cell Biol 12 326-333 (2000 Jan 1).

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10006515-96WELL
HAT Assay Buffer (5X)	1 ea
HAT Acetyl CoA	1 ea
Histone Acetyltransferase (pCAF)	1 ea
HAT Peptide	1 ea
HAT Stop Reagent	1 ea
HAT Developer	1 ea
96-Well Solid Plate (white)

Size	Global Purchasing
96 wells

Description

Cayman’s Histone Acetyltransferase (HAT) Inhibitor Screening Assay Kit provides a fast, fluorescence-based method for evaluating pCAF HAT inhibitors. The procedure requires only three easy steps, all performed in the same microwell plate. In the first step of the protocol, HAT is incubated with acetyl-CoA and the histone H3 peptide. During this time, HAT catalyzes the enzymatic transfer of acetyl groups from acetyl-CoA to the H3 peptide producing an acetylated peptide and CoASH. Following addition of isopropanol to stop the enzymatic reaction, CPM is added to the wells of the plate. CPM reacts with the free thiol groups present on CoASH forming a highly fluorescent product that is detected using excitation and emission wavelengths of 360-390 and 450-470 nm, respectively.

下一个：Glycolysis Cell-Based Assay Kit上一个：HDAC1 Inhibitor Screening Assay Kit

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