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Cat. Number
066086747618752
Chemical Name
Glycolysis Cell-Based Assay Kit
References
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Griffin, J.L., and Shockcor, J.P. Metabolic profiles of cancer cells. Cancer 4 551-561 (2004).

McCarty, M.F., and Whitaker, J. Manipulating tumor acidification as a cancer treatment strategy. Altern Med Rev 15(3) 264-272 (2010).

Barger, J.F., and Plas, D.R. Balancing biosynthesis and bioenergetics: Metabolic programs in oncogenesis. Endocrin-Related Cancer 17 R287-R304 (2010).

Demetrius, L.A., Coy, J.F., and Tuszynski, J.A. Cancer proliferation and therapy: The Warburg effect and quantum metabolism. Theoretical Biology and Medical Modeling 7 2-14 (2010).

Kondoh, H., Lleonart, M.E., Nakashima, Y., et al. A common metabolic profile shared between murine ES cells and primary cells bypassing senescence. Medical Hypothesis and Research 4(1) 29-36 (2008).

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600450-1ea
Cell-Based Assay Buffer Tablet 1 ea
Glycolysis Assay Substrate 250 µL
Glycolysis Assay Enzyme Mixture 2 × 1 ea
Glycolysis Assay L-Lactate Standard 100 µL
Glycolysis Assay Cofactor 250 µL
Size Global Purchasing
1 ea  

Description

Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting extracellular L-lactate, the end product of glycolysis, in cultured cells. In the assay, lactate dehydrogenase catalyzes the oxidation of lactate to pyruvate, in which the formed NADH reduces a tetrazolium substrate (INT) to a highly-colored formazan which absorbs strongly at 490-520 nm. The amount of formazan produced is proportional to the amount of lactate released into the culture medium and can be used as an indicator of the cellular glycolytic rate. This single step, no-wash assay can be adapted to high-throughput screening for glycolysis regulators at the cellular level. Furthermore, the non-invasive sampling leaves the cells intact and thus allows multiplex testing for additional markers.
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