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Cat. Number
065969285808040
Chemical Name
MTT Cell Proliferation Assay Kit
References
Stability 6 months
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Šulic, S., Panic, L., Ðikic, I., et al. Deregulation of cell growth and malignant transformation. Croat Med J 46(4) 622-638 (2005).

Francoeur, A., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3 19-25 (1996).

Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11 127-152 (2005).

10009365-2500DTN
Cell-Based Assay Buffer Tablet 1 ea
MTT Reagent 5 ea
Crystal Dissolving Solution 5 ea
10009365-500DTN
Cell-Based Assay Buffer Tablet 1 ea
MTT Reagent 1 ea
Crystal Dissolving Solution 1 ea
Size Global Purchasing
480 wells  
2,400 wells  

Description

Cayman’s MTT Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation. In this assay, MTT is taken up by cells through the plasma membrane potential and then reduced to formazan by intracellular NAD(P)H-oxidoreductases.1 The assay can be performed in 3-4 hours with no wash steps in a 96 well plate format.

1 Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11 127-152 (2005).

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