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Isotype Control：PerCP/Cyanine5.5 Mouse IgG2b, κ Isotype Ctrl Verified Reactivity：Human Reported Reactivity：Chimpanzee Antibody Type：Monoclonal Host Species：Mouse Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation：The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions. Concentration：Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) Storage & Handling：The CD45RA antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application：FC - Quality tested Recommended Usage：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. Excitation Laser：Blue Laser (488 nm) Application Notes：Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16. Additional Product Notes：BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions. Application References：
(PubMed link indicates BioLegend citation) Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block) Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F) Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P) Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed Palendira U, et al. 2008. Blood (FC) PubMed Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
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Ogonek J, et al. 2017. J Immunol. 10.4049/jimmunol.1601992. PubMed Maehara T, et al. 2018. Life Sci Alliance. 1:e201800050. PubMed Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed Liu Y, et al. 2018. JCI Insight. 3:. PubMed Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed Qu K et al. 2017. Cancer cell. 32(1):27-41 . PubMed Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed
RRID AB_893358 (BioLegend Cat. No. 304121)
AB_893357 (BioLegend Cat. No. 304122)
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