Isotype Control：APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl

• Verified Reactivity：Human, Cynomolgus, Rhesus

• Reported Reactivity：African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey

• Antibody Type：Monoclonal

• Host Species：Mouse

• Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

• Preparation：The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.

• Concentration：Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

• Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

• Application：FC - Quality tested

• Recommended Usage：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

• Excitation Laser：Red Laser (633 nm)

• Application Notes：Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

• Additional Product Notes：BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

• Application References：(PubMed link indicates BioLegend citation)

1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.

2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.

3. Evans RL, et al. 1981. Immunol. 78:544.

4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.

5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed

6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)

7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)

8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed

10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

• Product Citations

• Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-11056-9. PubMed

1. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed

2. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed

3. Washburn ML, et al. 2019. J Immunol. 203:1897. PubMed

4. Wang B, et al. 2018. Mol Ther Nucleic Acids. 0.548611111. PubMed

5. Bochem J, et al. 2019. PLoS One. 14:e0221301. PubMed

6. Hassounah NB, et al. 2019. Cancer Immunol Immunother. 68:407. PubMed

7. de Macedo Abdo L, et al. 2020. Oncoimmunology. 9:1752592. PubMed

8. Chiu YL, et al. 2018. Immun Ageing. 15:27. PubMed

9. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed

10. Pucino V, et al. 2020. Cell Metabolism. 30(6):1055-1074.e8.. PubMed

11. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed

12. See More

• RRID

• AB_2044005 (BioLegend Cat. No. 344713)
  AB_2044006 (BioLegend Cat. No. 344714)