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Cat. Number

066240659140629

Chemical Name

7-AAD Cell Viability Assay Kit

References

Synonyms	7-Amino Actinomycin D Cell Viability Kit
Stability	1 year
Storage	4°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Hynes, J., Floyd, S., Soini, A.E., et al. Fluorescence-based cell viability screening assays using water-soluble oxygen probes. J Biomol Screen 8 264-272 (2003).

Coder, D.M. Assessment of cell viability. Curr Protoc Cytom 9.2.1-9.2.14 (1997).

10009856-1ea
Cell-Based Assay Buffer Tablet	4 ea
Cell-Based Assay 7-AAD Staining Stock Solution (1,000X)	1 ea
7-AAD Assay Fixative/Actinomycin D Solution	1 ea

Size	Global Purchasing
1 ea

Description

Evaluation of cell viability is essential in cell biology and drug discovery. Cayman’s 7-AAD Cell Viability Assay Kit employs 7-AAD as a fluorescent label for dead cells. 7-AAD is a fluorescent dye which is excluded from live cells but penetrates dead or damaged cells to label DNA. Although 7-AAD fluorescence is less intense than that of propidium iodide, it exhibits a higher wavelength emission maximum (excitation at 488 nm, emission at 650 nm) and thus has minimal spectral overlap with PE or FITC. This makes 7-AAD preferable as a viability marker when FITC and/or PE are used simultaneously to label surface or intracellular antigens. A fixative/actinomycin D solution is included in the kit for cell fixation and blocking, making subsequential immunostaining of surface/intracellular antigens possible. This kit provides a convenient tool to quantify cytotoxic effects of environmental toxins or drug candidates.

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