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Cat. Number
066056442792403
Chemical Name
Human Therapeutic IgG4 EIA Kit
References
Synonyms
  • Human IgG4 Immunometric ELISA
Stability 6 months
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Chan, A.C., and Carter, P.J. Therapeutic antibodies for autoimmunity and inflammation. Nat Rev Immunol 10 301-16 (2010).

Salfeld, J.G. Isotype selection in antibody engineering. Nat Biotech 25 1369-1372 (2007).

Yang, J., Ng, C., Lowman, H., et al. Quantitative determination of humanized monoclonal antibody rrhuMAb2H7 in cynomolgus monkey serum using a generic immunoglobulin pharmacokinetic (GRIP) assay. J Immunol Methods 335(1-2) 8-20 (2008).

Stubenrauch, K., Wessels, U., and Lenz, H. Evaluation of an immunoassay for human-specific quantitation of therapeutic antibodies in serum samples from non-human primates. J Pharm Biomed Anal 49(4) 1003-8 (2009).

van Dijk, M.A., and van de Winkel, J.G. Human antibodies are next generation therapeutics. Curr Opin Chem Biol 5(4) 368-74 (2001).

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500920-96well
HRP Stop Solution 12 mL
96-Well Cover Sheet
Polysorbate 20 1 ea
Blue EIA Kit Box (96 Well)
Immunoassay Buffer B Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
TMB Substrate Solution 1 ea
Sheep Anti-Human IgG/HRP Detection Antibody 1.2 mL
Anti-Human IgG4 Precoated 96-Well Strip Plate 1 ea
IgG4 (human) EIA Standard 1 ea
Size Global Purchasing
96 wells  

Description

Human therapeutic antibodies have become increasingly common components of early drug discovery and development portfolios in the pharmaceutical and biotech industries. As part of preclinical toxicology assessment of these agents, they are routinely tested in non-human primates, primarily in rhesus or cynomolgus monkeys. In order to assess the pharmacokinetics of the human antibodies in monkey serum, it is necessary to have an assay that is capable of distinguishing the experimentally-introduced human IgG from the endogenous monkey IgG. Cayman’s IgG4 (human) Pharmacokinetic EIA Kit is an immunometric assay which can be used to measure human IgG4 in monkey or rodent plasma and serum without prior sample purification. The standard curve spans the range of 0-500 ng/ml, with a LLOQ of 7.8 ng/ml. The assay has been validated in serum from cynomolgus monkeys.
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