JMJD2A Inhibitor Screening Assay Kit_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

服务热线：13917250134「同微信」

您的位置：首页>>分析化学>>JMJD2A Inhibitor Screening Assay Kit

产品中心

/ Products Classification 点击展开+

Cat. Number

066052578739276

Chemical Name

JMJD2A Inhibitor Screening Assay Kit

References

Synonyms	Jumonji Domain Containing 2A Inhibitor Screening Assay Kit
Stability	6 months
Storage	-80°C
Shipping	Dry ice in continental US; may vary elsewhere

Background Reading

Tsukada, Y., Fang, J., Erdjument-Bromage, H., et al. Histone demethylation by a family of JmjC domain-containing proteins. Nature 439(16) 811-816 (2006).

Couture, J., Collazo, E., Ortiz-Tello, P.A., et al. Specificity and mechanism of JMJD2A, a trimethyllysine-specific histone demethylase. Nat Struct Mol Biol 14(8) 689-695 (2007).

Shin, S., and Janknecht, R. Activation of androgen receptor by histone demethylases JMJD2A and JMJD2D. Biochem Biophys Res Commun 359 742-746 (2007).

Li, Q., Sritharathikhun, P., and Motomizu, S. Development of novel reagent for Hantzsch reaction for the determination of formaldehyde by spectrophotometry and fluorometry. Anal Sci 23 413-417 (2007).

Rose, N.R., Woon, E.C.Y., Kingham, G.L., et al. Selective inhibitors of the JMJD2 histone demethylases: Combined nondenaturing mass spectrometric screening and crystallographic approaches. J Med Chem 53 1810-1818 (2010).

Strahl, B.D., and Allis, D. The language of covalent histone modifications. Nature 403 41-45 (2000 Jan 6).

Zhang, D., Yoon, H., and Wong, J. JMJD2A is a novel N-CoR-interacting protein and is involved in repression of the human transcription factor achaete scute-like homologue 2 (ASCL2/Hash2). Mol Cell Biol 25(15) 6404-6414 (2005).

Gray, S.G., Iglesias, A.H., Lizcano, F., et al. Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein. J Biol Chem 280(31) 28507-28518 (2005).

Katoh, M., and Katoh, M. Identification and characterization of JMJD2 family genes in silico. Int J Oncol 24 1623-1628 (2004).

Schneider, J., and Shilatifard, A. Histone demethylation by hydroxylation: Chemistry in action. ACS Chem Biol 1(2) 75-81 (2006).

Rose, N.R., Ng, S.S., Mecinovic, J., et al. Inhibitor scaffolds for 2-oxoglutarate-dependent histone lysine demethylases. J Med Chem 51 7053-7056 (2008).

Show all 11 Hide all but first 3

700360-96well
96-Well Cover Sheet
Half Volume 96-Well Solid Plate (black)
JMJ Assay Buffer	10 mL
JMJ Peptide	1.2 mL
JMJD2A (human recombinant) Assay Reagent	2 × 150 µL
JMJ Ammonium Acetate	5 mL
JMJ Formaldehyde Detector	150 mg
JMJ DMSO	2 mL

Size	Global Purchasing
96 wells

Description

Jumonji Domain Containing 2A (JMJD2A) is a Jumonji C (JmjC) histone demethylase that catalyzes the demethylation of di- and trimethylated lysine 9 and lysine 36 of histone H3. The development of JmjC-specific inhibitors will be an important tool for studying the functions of the various JmjC enzymes. Cayman’s JMJD2A Inhibitor Screening Assay Kit provides a convenient fluorescence-based method for screening JMJD2A inhibitors. The assay is based on the multistep reaction in which JMJD2A first produces formaldehyde during the demethylation of the trimethylated peptide substrate, histone H3 trimethyl lys9, with the concomitant oxidation/decarboxylation of 2-oxoglutarate. The detection reaction involves the cyclization between formaldehyde and acetoacetanilide in the presence of ammonia. The resulting fluorescent product is analyzed using an excitation wavelength between 365-375 nm and an emission wavelength between 465-475 nm.

下一个：Human Therapeutic IgG4 EIA Kit上一个：JMJD2D Inhibitor Screening Assay Kit

联系我们

电话：021-60498804
邮箱：info@e-biochem.com
地址：上海市徐汇区漕溪路258弄27号航星商务楼2-404

扫一扫，关注我们

惠诚品牌

产品中心: 分子生物; 分析化学; 生化试剂; 仪器耗材

企业动态

技术资料

在线咨询联系方式二维码

服务热线

021-60498804

扫一扫，关注我们