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Cat. Number

065997512417542

Chemical Name

Lysosome/Cytotoxicity Dual Staining Kit

References

Stability	1 year
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Ishiguro, K., Ando, T., and Goto, H. Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells. Biotechniques 45(4) 465-468 (2008).

Fehrenbacher, N., and Jäättelä, M. Lysosomes as targets for cancer therapy. Cancer Res 65(8) 2993-2995 (2005).

Schneider, L., and Zhang, J. Lysosomal function in macromolecular homeostasis and bioenergetics in Parkinson’s disease. Mol Neurodegener 5 14 (2010).

Zhang, L., Sheng, R., and Qin, Z. The lysosome and neurodegenerative diseases. Acta Biochim Biophys Sin 41(6) 437-445 (2009).

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600310-1ea
Cell-Based Assay Buffer Tablet	5 ea
Cell-Based Propidium Iodide Solution	1 ea
Chloroquine Positive Control (25 mM)	1 ea
Shrimp Kit Box (96 Well)
NBD-PZ Stock Solution	100 µL

Size	Global Purchasing
1 ea

Description

Cayman’s Lysosome/Cytotoxicity Dual Staining Kit is a tool for studying lysosome function at the cellular level. The kit employs 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole (NBD-PZ), which is membrane permeable and reacts with carboxylic acids in the acidic luminal environment of lysosomes,¹ as a probe for the detection of lysosomes in cultured cells. Propidium iodide is used as a marker of cell death. Chloroquine, a known inhibitor of lysosome function, is included as a positive control. The kit provides sufficient reagents to effectively treat/stain up to 10 plates worth of cells irrespective of the number of wells/plate.

1 Ishiguro, K., Ando, T., and Goto, H. Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells. Biotechniques 45(4) 465-468 (2008).

下一个：LSD1 Inhibitor Screening Assay Kit上一个：Melanocortin-3 Receptor STEP Reporter Assay Kit (Luminescence)

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