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Cat. Number

065959600116562

Chemical Name

Multi-Parameter Apoptosis Assay Kit

References

Stability	1 year
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Schiller, M., Bekerdjian-Ding, I., Heyder, P., et al. Autoantigens are translocated into small apoptotic bodies during early stages of apoptosis. Cell Death Differ 15 183-191 (2008).

van Engeland, M., Nieland, L.J.W., Ramaekers, F.C.S., et al. Annexin V-affinity assay: A review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry 31 1-9 (1998).

Elmore, S. Apoptosis: A review of programmed cell death. Toxicol Pathol 35(4) 495-516 (2007).

600330-1ea
Shrimp Kit Box (96 Well)
Cell-Based Assay 7-AAD Staining Stock Solution (1,000X)	1 ea
Cell-Based Annexin V FITC	50 µL
Cell-Based Assay Annexin V Binding Buffer (10X)	50 mL
Cell-Based Assay TMRE	100 µL
Cell-Based Assay Hoechst Dye	50 µL

Size	Global Purchasing
5 x 96 wells

Description

Cellular events in apoptosis include loss of cell membrane asymmetry, cell shrinkage, membrane blebbing, nuclear fragmentation, and chromatin condensation. Cayman’s Multi-Parameter Apoptosis Assay Kit includes four different reagents for monitoring several events in apoptosis. It employs FITC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells, TMRE as a probe for mitochondrial membrane potential, 7-AAD as an indicator of membrane permeability/cell viability, and Hoechst Dye to demonstrate nuclear morphology. The kit allows phenotypic characterization of different cell death parameters at a single-cell level. The assay can be adapted to high content screening with appropriate equipment. The reagents provided in the kit are sufficient to run 100 samples when using flow cytometry, or 500 samples when using a 96-well plate format.

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