| References |
| Synonyms |
- PAF-AH Inhibitor Screening Assay Kit
- Lp-PLA2 Inhibitor Screening Assay Kit
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| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
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Background Reading
Prescott, S.M., Zimmerman, G.A., and McIntyre, T.M. Platelet-activating factor. J Biol Chem 265 17381-17384 (1990).
Snyder, F. Platelet-activating factor and related acetylated lipids as potent biologically active cellular mediators. Am J Physiol Cell Physiol 259 C697-C708 (1990).
Caslake, M.J., and Packard, C.J. Lipoprotein-associated phospholipase A2 (platelet-activating factor acetylhydrolase) and cardiovascular disease. Curr Opin Lipidol 14 347-352 (2003).
Aarsman, A.J., Neys, F.W., and van den Bosch, H. Catabolism of platelet-activating factor and its acyl analog. Differentiation of the activities of lysophospholipase and platelet-activating-factor acetylhydrolase. Eur J Biochem 200 187-193 (1991).
Kell, P.J., Creer, M.H., Crown, K.N., et al. Inhibition of platelet-activating factor (PAF) acetylhydrolase by methyl arachidonyl fluorophosphonate potentiates PAF synthesis in thrombin-stimulated human coronary artery endothelial cells. J Pharmacol Exp Ther 307(3) 1163-1170 (2003).
Stafforini, D.M., Prescott, S.M., and McIntyre, T.M. Human plasma platelet-activating factor acetylhydrolase. J Biol Chem 262 4223-4230 (1987).
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| Size |
Global Purchasing |
| 96 wells |
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Description
Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of stimulated cells. The biological effects of PAF include activation of platelets, polymorphonuclear leukocytes, monocytes, and macrophages. PAF also increases vascular permeability, decreases cardiac output, induces hypotension, and stimulates uterine contraction.1 PAF has been implicated in pathological processes, such as inflammation and allergy.2 PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). PAF-AHs are located intra- and extra-cellularly (e.g., cytosolic and plasma). Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2 position and is associated with lipoproteins.3 Recently, plasma PAF-AH has been linked to atherosclerosis and may be a positive risk factor for coronary heart disease in humans.4 The Cayman Chemical PAF Acetylhydrolase Inhibitor Screening Assay uses 2-thio PAF as a substrate for PAF-AH.5 Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB; Ellman’s reagent). The PAF Acetylhydrolase Inhibitor Screening assay includes human plasma PAF-AH and is a time saving tool for screening vast numbers of inhibitors.
1
Prescott, S.M., Zimmerman, G.A., and McIntyre, T.M. Platelet-activating factor. J Biol Chem 265 17381-17384 (1990).
2
Snyder, F. Platelet-activating factor and related acetylated lipids as potent biologically active cellular mediators. Am J Physiol Cell Physiol 259 C697-C708 (1990).
3
Stafforini, D.M., Prescott, S.M., and McIntyre, T.M. Human plasma platelet-activating factor acetylhydrolase. J Biol Chem 262 4223-4230 (1987).
4
Caslake, M.J., and Packard, C.J. Lipoprotein-associated phospholipase A2 (platelet-activating factor acetylhydrolase) and cardiovascular disease. Curr Opin Lipidol 14 347-352 (2003).
5
Aarsman, A.J., Neys, F.W., and van den Bosch, H. Catabolism of platelet-activating factor and its acyl analog. Differentiation of the activities of lysophospholipase and platelet-activating-factor acetylhydrolase. Eur J Biochem 200 187-193 (1991).
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