Verified Reactivity：Human

Reported Reactivity：Chimpanzee

Antibody Type：Monoclonal

Host Species：Mouse

Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation：The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

Concentration：Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application：FC - Quality tested

Recommended Usage：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser：Blue Laser (488 nm)

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes：Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes:BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References:(PubMed link indicates BioLegend citation)

1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)

2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)

3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)

4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)

5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)

6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)

7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)

8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed

9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)

10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)

12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed

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Product Citations

1. Taraldsrud E, et al. 2017. Journal of Autoimmunity. 10.1016/j.jaut.2017.04.004. PubMed

2. Verma K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183828. PubMed

3. Gladkikh A, et al. 2017. Cancer Med. . 10.1002/cam4.1257. PubMed

4. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed

5. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed

6. Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed

7. Meraviglia S, et al. 2017. Oncoimmunology. 6:e1347742. PubMed

8. Leone DA, et al. 2017. J Immunol. 199:531. PubMed

9. Iio K, et al. 2019. Sci Rep. 9:813. PubMed

10. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed

11. Goel G, et al. 2019. Sci Adv. 5:eaaw7756. PubMed

12. Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed

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RRID

AB_439780 (BioLegend Cat. No. 300419)
AB_439781 (BioLegend Cat. No. 300420)