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Cat. Number
302626
Chemical Name
302626 PerCP/Cyanine5.5 anti-human CD25 Antibody
Qty 1
100tests
Storage condition
2°C and 8°C
References
  • Isotype Control:PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl

  • Verified Reactivity:Human

  • Reported Reactivity:Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus

  • Antibody Type:Monoclonal

  • Host Species:Mouse

  • Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

  • Preparation:The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.

  • Concentration:Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

  • Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

  • Application:FC - Quality tested

  • Recommended Usage:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

  • * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

  • Excitation Laser:Blue Laser (488 nm)

  • Application Notes:Additional reported applications include: immunocytochemistry3.

  • Additional Product Notes:BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

  • Application References:
    (PubMed link indicates BioLegend citation)

    1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.

    2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed

    3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed

  • Product Citations

    1. Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed

    2. Chen EW, et al. 2019. Front Immunol. 10:1718. PubMed

  • RRID

  • AB_2125479 (BioLegend Cat. No. 302625)
    AB_2125478 (BioLegend Cat. No. 302626)


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