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Cat. Number
Cyanine5 alkyne
Chemical Name
Cyanine5 alkyne
Mol. Formula
C35H42ClN3O
Mol. Weight
556.18
Appearance
dark blue powder
Storage condition
-20°C
References

Cyanine5 alkyne for Click Chemistry, an analog of Cy5® alkyne. With this product, deeply colored, and photostable Cyanine5 fluorophore can be attached to various molecules via Click Chemistry reaction with azides.

This alkyne is non-water soluble, but it can be dissolved in DMF or DMSO prior to reaction, and added to aqueous reaction mixture. With our labeling protocol, the labeling reaction is very efficient, and high-yielding.

Various substrates bearing azides can be used for the labeling, including azido-labeled biomolecules, polymers, and solid surfaces.

 

Product citations

  1. Sacoman, J.L.; Dagda, R.Y.; Burnham-Marusich, A.R.; Dagda, R.K.; Berninsone, P.M. Mitochondrial O-GlcNAc transferase (mOGT) regulates mitochondrial structure, function and survival in HeLa cells. Journal of Biological Chemistry, in press. doi:10.1074/jbc.M116.726752

  2. Wallat, J.D.; Czapar, A.E.; Wang, C.; Wen, A.M.; Wek, K.S.; Yu, X.; Steinmetz, N.F.; Pokorski, J.K. Optical and Magnetic Resonance Imaging Using Fluorous Colloidal Nanoparticles. Biomacromolecules2017, 18(1), 103–112. doi:10.1021/acs.biomac.6b01389

  3. Ponomarenko, A.I.; Brylev, V.A.; Sapozhnikova, K.A.; Ustinov, A.V.; Prokhorenko, I.A.; Zatsepin, T.S.; Korshun, V.A. Tetrahedral DNA conjugates from pentaerythritol-based polyazides. Tetrahedron2016, 72(19), 2386–2391. doi: 10.1016/j.tet.2016.03.051

  4. Burnham-Marusich, A.R.; Plechaty, A.M.; Berninsone, P.M. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis2014, 35(18), 2621-2625. doi: 10.1002/elps.201400241

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