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This kit contains four separate vials of the following compounds in the amounts indicated:
DLM-584 Succinate (U-D4), 2.5g
DLM-4646 Alpha-Keto Isovalerate (U-D7), 0.2 g
DLM-141 Isoleucine (U-D10), 0.06g
CDLM-8649 Alanine (2-D, 3-13C), 0.8 g
The materials in this kit are to be used in conjunction with 1L of deuterated minimal media1,2. 1L of minimal media that is suitable for use with this product typically contains 2 g of glucose-D7, 1 g of ammonium salt (either 15N labeled or unlabeled, depending on preference of the user), and 11.3 g of M9 salts in D2O.
1 Ayala, I.; Sounier, R.; Use, N.; Gans, P.; Boisbouvier, J. J. Biomol. NMR 2009, 43, 111-9.
2 Isaacson, R.L.; Simpson, P.J.; Liu, M.; Cota, E.; Zhange, X.; Freemont, P.; Matthews, S. J Am Chem Soc 2007, 129, 15428-9.
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