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Cat. Number
996828399288322
Chemical Name
BRDT bromodomain 1 TR-FRET Assay Kit
References
Synonyms
  • BRD6
  • BRD-containing protein testis specific
  • CT9
  • RING3-like protein
  • Cancer/testis antigen 9
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Filippakopoulos, P., Qi, J., Picaud, S., et al. Selective inhibition of BET bromodomains. Nature 468(7327) 1067-1073 (2010).

Hewings, D.S., Wang, M., Philpott, M., et al. 3,5-Dimethylisoxazoles act as acetyl-lysine-mimetic bromodomain ligands. J Med Chem 1-30 (2011).

Philpott, M., Yang, J., Tumber, T., et al. Bromodomain-peptide displacement assays for interactome mapping and inhibitor discovery. Mol BioSyst 7(10) 2899-2908 (2011).

Delmore, J.E., Issa, G.C., Lemieux, M.E., et al. BET bromodomain inhibition as a therapeutic strategy to target c-Myc. Cell 146(6) 904-917 (2011).

Zhang, J., Chung, T.D.Y., and Oldenburg, K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4(2) 67-73 (1999).

Weidner-Glunde, M., Ottinger, M., and Schulz, T.F. WHAT do viruses BET on? Front Biosci 15 537-549 (2010).

Day, A., Chitsaz, F., Abbasi, A., et al. The double bromodomain protein Brd4 binds to acetylated chromatin during interphase and mitosis. Proc Natl Acad Sci USA 100(15) 8758-8763 (2003).

Umehara, T., Nakamura, Y., Jang, M.K., et al. Structural basis for acetylated histone H4 recognition by the human BRD2 bromodomain. J Biol Chem 2855(10) 7610-7618 (2010).

Mujtaba, S., Zeng, L., and Zhou, M. Structure and acetyl-lysine recognition of the bromodomain. Oncogene 26 5521-5527 (2011).

Florence, B., and Faller, D.V. You bet-cha: A novel family of transcriptional regulators. Front Biosci 6 D1008-D1018 (2011).

LeRoy, G., Rickards, B., and Flint, S.J. The double bromodomain proteins Brd2 and Brd3 couple histone acetylation to transcription. Mol Cell 30(1) 51-60 (2008).

Liu, Y., Wang, X., Zhang, J., et al. Structural basics and binding properties of the second bromodomain of Brd4 with acetylated histone tails. Biochem 47 6403-6417 (2008).

Chung, C.W., Coste, H., White, J.H., et al. Discovery and characterization of small molecule inhibitors of the BET family bromodomains. J Med Chem 54(11) 3827-3838 (2011).

Hargreaves, D.C., Horng, T., and Medzhitov, R. Control of inducible gene expression by signal-dependent transcriptional elongation. Cell 138(1) 129-45 (2009).

Umehara, T., Nakamura, Y., Wakamori, M., et al. Structural implications for K5/K12-di-acetylated histone H4 recognition by the second bromodomain of BRD2. FEBS Lett 584(18) 3901-3908 (2010).

Nicodeme, E., Jeffrey, K.L., Schaefer, U., et al. Suppression of inflammation by a synthetic histone mimic. Nature 468(7327) 1119-23 (2010).

Mertz, J.A., Conery, A.R., Bryant, B.M., et al. Targeting MYC dependence in cancer by inhibiting BET bromodomains. Proc Natl Acad Sci USA 108(40) 16669-16674 (2011).

Dawson, M.A., Prinjha, R.K., Dittmann, A., et al. Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia. Nature 478 529-533 (2011).

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600650-1920well
Foil Plate Covers
384-Well Solid Plate (low volume; black)
TR-FRET Assay Buffer (10X) 10 mL
TR-FRET Assay Buffer Additive 1 g
(+)-JQ1 Positive Control 5 × 8 nmol
BRDT bromodomain 1 Europium Chelate 5 × 420 Well
BRDT bromodomain 1 Ligand/APC Acceptor Mixture 5 × 420 Well
600650-384well
Foil Plate Covers
384-Well Solid Plate (low volume; black)
TR-FRET Assay Buffer (10X) 2 mL
TR-FRET Assay Buffer Additive 200 mg
(+)-JQ1 Positive Control 8 nmol
BRDT bromodomain 1 Europium Chelate 420 Well
BRDT bromodomain 1 Ligand/APC Acceptor Mixture 420 Well
600650-9600well
Foil Plate Covers
384-Well Solid Plate (low volume; black)
TR-FRET Assay Buffer (10X) 5 × 10 mL
TR-FRET Assay Buffer Additive 5 × 1 g
(+)-JQ1 Positive Control 5 × 40 nmol
BRDT bromodomain 1 Europium Chelate 5 × 2100 Well
BRDT bromodomain 1 Ligand/APC Acceptor Mixture 5 × 2100 Well
Size Global Purchasing
384 wells  
1920 wells  
9600 wells  

Description

Bromodomains recognize acetylated lysine residues and recruit regulatory complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. The isolated individual or tandem bromodomains of many BET family members, including BRD2, BRD3, BRD4, and BRDT, have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of bromodomain interactions hold promise as useful therapeutics for human disease. Cayman’s BRDT Bromodomain 1 TR-FRET Assay Kit is a homogeneous, Time-Resolved Fluorescence Resonance Energy Transfer assay method amenable to rapid characterization of inhibitors of bromodomain/acetylated peptide interaction in a high throughput format. This screening assay kit is robust, displaying a Z’ >0.6.
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