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Cat. Number
066067576744452
Chemical Name
Human Serum Albumin EIA Kit
References
Synonyms
  • Human Serum Albumin EIA Kit
  • HSA ELISA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
urine Human Serum Albumin 100%

Background Reading

Hovind, P., Tarnow, L., Rossing, P., et al. Predictors for the development of microalbuminuria and macroalbuminuria in patients with type 1 diabetes: inception cohort study. BMJ 328 1105-1110 (2004).

Damsgaard, E.M., Froland, A., Jorgensen, O.D., et al. Microalbuminuria as predictor of increased mortality in elderly people. Br Med J 300 297-300 (1990).

Brinkman, J.W., de Zeeuw, D., Duker, J.J., et al. Falsely low urinary albumin concentrations after prolonged frozen storage of urine samples. Clin Chem 51(11) 2181-2183 (2005).

Justesen, T.I., Petersen, J.L.A., Ekbom, P., et al. Albumin-to-creatinine ratio in random urine samples might replace 24-h urine collections in screening for micro- and macroalbuminuria in pregnant woman with type 1 diabetes. Diabetes Care 29(4) 924-925 (2006).

Ruggenenti, P., Gaspari, F., Perna, A., et al. Cross sectional longitudinal study of spot morning urine protein: Creatinine ratio, 24 hour urine protein excretion rate, glomerular filtration rate, and end stage renal failure in chronic renal disease in patients without diabetes. BMJ 316 504-509 (1998).

Show all 5 Hide all but first 3
500340-96SOLID
Immunoassay Buffer A Concentrate (10X) 2 × 10 mL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
Wash Buffer Concentrate (400X) 5 mL
Human Serum Albumin AChE Tracer 100 dtn
Human Serum Albumin EIA Monoclonal Antibody 100 dtn
Human Serum Albumin EIA Standard 1 ea
500340-480well
Immunoassay Buffer A Concentrate (10X) 4 × 10 mL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
Wash Buffer Concentrate (400X) 12.5 mL
Human Serum Albumin AChE Tracer 500 dtn
Human Serum Albumin EIA Monoclonal Antibody 500 dtn
Human Serum Albumin EIA Standard 1 ea
500340-96well
Immunoassay Buffer A Concentrate (10X) 2 × 10 mL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
Wash Buffer Concentrate (400X) 5 mL
Human Serum Albumin AChE Tracer 100 dtn
Human Serum Albumin EIA Monoclonal Antibody 100 dtn
Human Serum Albumin EIA Standard 1 ea
5000340-480SOLID
Immunoassay Buffer A Concentrate (10X) 4 × 10 mL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
Wash Buffer Concentrate (400X) 12.5 mL
Human Serum Albumin AChE Tracer 500 dtn
Human Serum Albumin EIA Monoclonal Antibody 500 dtn
Human Serum Albumin EIA Standard 1 ea
Size Global Purchasing
96 solid wells  
96 strip wells  
480 solid wells  
480 strip wells  

Description

Limit of detection: 80% B/B0: 65 pg/ml · Sensitivity: 50% B/B0: 250 ng/ml · The measurement of albumin in urine is well established clinically as an indicator of renal function. Approximately fifty percent of human blood serum protein is albumin. Albumin (human serum) (HSA) has many important physiological functions including maintaining osmotic pressure, buffering pH of the blood, and the transport of hormones, fatty acids, and other molecules throughout the body. Elevated levels of albumin (microalbuminuria and macroalbuminuria) in the urine are an indication of various disease states including diabetic nephropathy, non-diabetic kidney disease, and cardiovascular morbidity and mortality.1,2,3 Normal urinary HSA levels of adults are less than 30 µg albumin per mg of creatinine. Microalbuminuria is defined as 30-300 µg albumin per mg of creatinine and Macroalbuminuria is defined as albumin levels greater than 300 µg/mg.4,5 Cayman’s HSA EIA Kit is a competitive assay that can be used for quantification of HSA in urine. The EIA typically displays an IC50 (50% B/B0) of aproximately 250 ng/ml and a detection limit (80% B/B0) of approximately 60 ng/ml.

1 Hovind, P., Tarnow, L., Rossing, P., et al. Predictors for the development of microalbuminuria and macroalbuminuria in patients with type 1 diabetes: inception cohort study. BMJ 328 1105-1110 (2004).

2 Damsgaard, E.M., Froland, A., Jorgensen, O.D., et al. Microalbuminuria as predictor of increased mortality in elderly people. Br Med J 300 297-300 (1990).

3 Brinkman, J.W., de Zeeuw, D., Duker, J.J., et al. Falsely low urinary albumin concentrations after prolonged frozen storage of urine samples. Clin Chem 51(11) 2181-2183 (2005).

4 Justesen, T.I., Petersen, J.L.A., Ekbom, P., et al. Albumin-to-creatinine ratio in random urine samples might replace 24-h urine collections in screening for micro- and macroalbuminuria in pregnant woman with type 1 diabetes. Diabetes Care 29(4) 924-925 (2006).

5 Ruggenenti, P., Gaspari, F., Perna, A., et al. Cross sectional longitudinal study of spot morning urine protein: Creatinine ratio, 24 hour urine protein excretion rate, glomerular filtration rate, and end stage renal failure in chronic renal disease in patients without diabetes. BMJ 316 504-509 (1998).

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