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Cat. Number

066044610392015

Chemical Name

LDH Cytotoxicity Assay Kit

References

Synonyms	Lactate Dehydrogenase Cytotoxicity Assay Kit
Stability	1 year
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Bonfoco, E., Krainc, D., Ankarcrona, M., et al. Apoptosis and necrosis: Two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc Natl Acad Sci USA 92 7162-7166 (1995).

Haslam, G., Wyatt, D., and Kitos, P.A. Estimating the number of viable animal cells in multi-well cultures based on their lactate dehydrogenase activities. Cytotechnology 32 63-75 (2000).

Wolterbeek, H.T., and van deer Meer, J.G.M. Optimization, application, and interpretation of lactate dehydrogenase meaurements in microwell determination of cell number and toxicity. Assay Drug Dev Technol 3(6) 675-682 (2005).

10008882-480WELL
LDH Diaphorase	5 ea
LDH NAD+ (100X)	5 ea
LDH Lactic Acid (100X)	5 ea
LDH Standard	1 ea
Cell-Based Assay Buffer Tablet	1 ea
LDH INT (100X)	5 ea

10008882-96WELL
LDH Diaphorase	1 ea
LDH NAD+ (100X)	1 ea
LDH Lactic Acid (100X)	1 ea
LDH Standard	1 ea
Cell-Based Assay Buffer Tablet	1 ea
LDH INT (100X)	1 ea

Size	Global Purchasing
96 wells
480 wells

Description

Cell death can occur either by apoptosis or by necrosis. Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability, whereas apoptosis involves an articulated breakdown of the cell into membrane-bound apoptotic bodies.¹ Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme that is released into the culture medium following loss of membrane integrity resulting from either apoptosis or necrosis. LDH activity, therefore, can be used as an indicator of cell membrane integrity and serves as a general means to assess cytotoxicity resulting from chemical compounds or environmental toxic factors. Cayman’s LDH Cytotoxicity Assay Kit measures LDH activity present in the culture medium using a coupled two-step reaction. In the first step, LDH catalyzes the reduction of NAD⁺ to NADH and H⁺ by oxidation of lactate to pyruvate. In the second step of the reaction, diaphorase uses the newly-formed NADH and H⁺ to catalyze the reduction of a tetrazolium salt (INT) to highly-colored formazan which absorbs strongly at 490-520 nm.

1 Bonfoco, E., Krainc, D., Ankarcrona, M., et al. Apoptosis and necrosis: Two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc Natl Acad Sci USA 92 7162-7166 (1995).

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