Lipoxygenase Inhibitor Screening Assay Kit_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

服务热线：13917250134「同微信」

您的位置：首页>>分析化学>>Lipoxygenase Inhibitor Screening Assay Kit

产品中心

/ Products Classification 点击展开+

Cat. Number

066020362794661

Chemical Name

Lipoxygenase Inhibitor Screening Assay Kit

References

Synonyms	LO Inhibitor Screening Assay Kit
Stability	1 year
Storage	4°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Nassar, G.M., Morrow, J.D., Roberts, L.J., et al. Induction of 15-lipoxygenase by interleukin-13 in human blood monocytes. J Biol Chem 269 27631-27634 (1994).

Samuelsson, B., Dahlén, S., Lindgren JÅ, et al. Leukotrienes and lipoxins: Structures, biosynthesis, and biological effects. Science 237 1171-1176 (1987).

Yamamoto, S. Mammalian lipoxygenases: Molecular structures and functions. Biochim Biophys Acta 1128 117-131 (1992).

Gaffney, B.J. Lipoxygenases: Structural principles and spectroscopy. Annu Rev Biophys Biomol Struct 25 431-459 (1996).

Show all 4 Hide all but first 3

760700-96WELL
96-Well Solid Plate (Colorimetric Assay)
Lipoxygenase Inhibitor Screening Assay Buffer (10X)	1 ea
Developing Reagent 1	1 ea
Developing Reagent 2	1 ea
Potassium Hydroxide	1 ea
15-Lipoxygenase Standard	1 ea
Arachidonic Acid (substrate)	1 ea
Linoleic Acid (substrate)	1 ea

Size	Global Purchasing
96 wells

Description

Lipoxygenases are members of a class of non-heme iron-containing dioxygenases that catalyze the addition of molecular oxygen to fatty acids containing a cis,cis-1,4-pentadiene system to give an unsaturated fatty acid hydroperoxide. In mammals, lipoxygenases carry out the first step in the arachidonic acid cascade.^1,2 5- and 15-LOs lead to the biologically active lipoxins, whereas 5-LO leads to 5,6-epoxy-leukotrienes which are involved in a variety of inflammatory responses, including neutrophil chemotaxis, vascular permeability, and smooth muscle contraction.³ In contrast, Nassar, et al., suggested that animal 15-LO products act as anti-inflammatory agents.⁴ This implies that the 5- and 15-LO pathways may play a role in regulating inflammation. To elucidate the role of each lipoxygenase, it is particularly important to develop inhibitors that are enzyme specific. This assay kit provides an accurate and convenient method for screening lipoxygenase inhibitors. This assay measures the hydroperoxides generated from the incubation of a lipoxygenase (5-, 12-, or 15-LO) with either arachidonic or linoleic acid.

1 Gaffney, B.J. Lipoxygenases: Structural principles and spectroscopy. Annu Rev Biophys Biomol Struct 25 431-459 (1996).

2 Yamamoto, S. Mammalian lipoxygenases: Molecular structures and functions. Biochim Biophys Acta 1128 117-131 (1992).

3 Samuelsson, B., Dahlén, S., Lindgren JÅ, et al. Leukotrienes and lipoxins: Structures, biosynthesis, and biological effects. Science 237 1171-1176 (1987).

4 Nassar, G.M., Morrow, J.D., Roberts, L.J., et al. Induction of 15-lipoxygenase by interleukin-13 in human blood monocytes. J Biol Chem 269 27631-27634 (1994).

下一个：Lipid Droplets Fluorescence Assay Kit上一个：LSD1 Inhibitor Screening Assay Kit

联系我们

电话：021-60498804
邮箱：info@e-biochem.com
地址：上海市徐汇区漕溪路258弄27号航星商务楼2-404

扫一扫，关注我们

惠诚品牌

产品中心: 分子生物; 分析化学; 生化试剂; 仪器耗材

企业动态

技术资料

在线咨询联系方式二维码

服务热线

021-60498804

扫一扫，关注我们